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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated IA-2/PTPRN Monoclona (CL488-66045)

    IF Staining of HepG2 using CL488-66045

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66045 (IA-2/PTPRN antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated IA-2/PTPRN Monoclona (CL488-66045)

    FC experiment of HEK-293 using CL488-66045

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IA-2/PTPRN (CL488-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Y79 cells using CoraLite® Plus 488-conjugated IA-2/PTPRN Monoclona (CL488-66045)

    FC experiment of Y79 using CL488-66045

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IA-2/PTPRN (CL488-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66045
Clone No.1D3F5

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

IA-2, PTPRN, 1D3F5, ICA512-transmembrane fragment, ICA512-N-terminal fragment

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293 cells, Y79 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66045 targets IA-2/PTPRN in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17909

Product name: Recombinant human PTPRN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 510-811 aa of BC007713

Sequence: WCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, N
Calculated molecular weight 105 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC007713
Gene Symbol IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_2883238
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16849
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IA-2/PTPRN antibody CL488-66045Download protocol
FC protocol for CL Plus 488 IA-2/PTPRN antibody CL488-66045Download protocol
Standard Protocols
Click here to view our Standard Protocols