Human gp130 ELISA Kit0 Publications

カタログ番号: KE00176

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human gp130 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
1.8 pg/mL

測定範囲:
78.1-5000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 89% 78%-98%
Cell culture supernatants 101% 93%-110%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 146.2 3.1 2.1
2 20 575.3 2.6 2.6
3 20 2,397.1 3.3 3.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 149.5 6.1 4.1
2 24 577.7 16.4 2.8
3 24 2,270.1 88.3 3.9

製品概要
KE00176 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The gp130 ELISA kit is to be used to detect and quantify protein levels of endogenous gp130. The assay recognizes human gp130. An antibody specific for gp130 has been pre-coated onto the microwells. The gp130 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human gp130 is added to detect the captured human gp130 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD130, CDw130, Glycoprotein 130, GP130, GP130 RAPS, IL 6 receptor subunit beta, IL 6R beta, IL 6R subunit beta, IL 6RB, IL6R beta, IL6ST, Membrane glycoprotein 130
背景説明

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC). These cytokines signal through the gp130/Jak/STAT pathway. Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells.


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