Human UCH-L1 ELISA Kit0 Publications

カタログ番号: KE00211

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human UCH-L1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell Lysates

アッセイ方法:
Sandwich

感度:
0.02 ng/mL

測定範囲:
0.156-10 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human Serum 92% 84%-99%
Cell Lysates 104% 72%-127%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 4.58 0.16 3.4
2 20 1.14 0.03 2.9
3 20 0.30 0.02 5.9

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 4.54 0.22 4.9
2 24 1.02 0.03 3.1
3 24 0.23 0.02 8.6

製品概要
KE00211 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The UCH-L1 ELISA kit is to be used to detect and quantify protein levels of endogenous UCH-L1. The assay recognizes human UCH-L1. An antibody specific for UCH-L1 has been pre-coated onto the microwells. The UCH-L1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human UCH-L1 is added to detect the captured human UCH-L1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1
背景説明

Ubiquitin C-terminal hydrolase 1 (UCHL1) , previously known as PGP9.5, is found primarily in neurons and neuroendocrine cells with a very high concentration in dendrites and perikarya. UCHL1 is a more recently recognized biomarker of neuronal injury. UCHL1 could be detected in the CSF and serum with a half-life of 7-9 hours after severe traumatic brain injury. Increased plasma concentrations of UCHL1 therefore reflect increased blood brain barrier permeability and destruction of neurons.


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