Human Total IL-18 ELISA Kit0 Publications

カタログ番号: KE00193

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human Total IL-18 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
0.3 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 109% 98%-127%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 475.7 22.1 4.7
2 20 112.5 3.6 3.2
3 20 27.3 1.4 5.1

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 506.3 49.1 9.7
2 24 114.3 9.6 8.4
3 24 28.0 1.5 5.5

製品概要
KE00193 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total IL-18 ELISA kit is to be used to detect and quantify protein levels of endogenous Total IL-18. The assay recognizes human Total IL-18. An antibody specific for Total IL-18 has been pre-coated onto the microwells. The Total IL-18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Total IL-18 is added to detect the captured human Total IL-18 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18
背景説明

IL-18 is a member of IL-1 superfamily of cytokines. IL-18 is expressed by many human lymphoid and nonlymphoid cells, and has an important role in inflammatory processes. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 synergistically with IL-12 contributes to Th1 differentiation and, therefore, is important in host defense mechanisms against intracellular bacteria, viruses, and fungi. The IL-18 cytokine increased expression of vascular cell adhesion molecule-1(VCAM1) and the adherence of melanoma cells. IL-18 plays a major role in atherosclerotic plaque destabilization leading to acute ischemic syndromes. IL-18 negatively regulate NAFLD/NASH progression, as well as multiple aspects of metabolic syndrome via modulation of the gut microbiota.


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