Human TNFRSF6B/DcR3 ELISA Kit0 Publications

カタログ番号: KE00188

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human TNFRSF6B/DcR3 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell lysates

アッセイ方法:
Sandwich

感度:
3.4 pg/mL

測定範囲:
39-2500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell lysates 84% 75%-96%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,145.1 27.5 2.4
2 20 264.4 5.0 1.9
3 20 62.8 2.3 3.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,200.1 24.8 2.1
2 24 262.6 6.1 2.3
3 24 55.6 2.3 4.2

製品概要
KE00188 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF6B/DcR3 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF6B/DcR3. The assay recognizes human TNFRSF6B/DcR3. An antibody specific for TNFRSF6B/DcR3 has been pre-coated onto the microwells. The TNFRSF6B/DcR3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TNFRSF6B/DcR3 is added to detect the captured human TNFRSF6B/DcR3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
DCR3, Decoy receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, TNFRSF6B, TR6
背景説明

Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), also identified as DcR3 or M68, is a secreted member of the Tumor Necrosis Factor (TNF) receptor superfamily, neutralizes three different TNF ligands: TNFS14/LIGHT, TNFSF15/TL1A and TNFSF6/FasL. TNFRSF6B binds to FasL and inhibits FasL-induced apoptosis. It can also bind to LIGHT and suppresse LIGHT-mediated apoptosis. Interaction of TL1A with DR3 promotes T cell expansion during an immune response. Expression of DcR3 is significantly elevated in patients with a range of cancers and autoimmune diseases.


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