Human TFPI ELISA Kit0 Publications

カタログ番号: KE00202

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human TFPI ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
7.2 pg/mL

測定範囲:
15.6-1000 pg/mL , 62.5-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 75%-122% 107%
Cell culture supernatants 72%-120% 89%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 23.3 2.2 9.4
2 20 75.7 5.4 7.1
3 20 312.9 15.6 5.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 22.9 1.5 6.6
2 24 66.8 5.6 8.5
3 24 314.2 18.1 5.8

製品概要
KE00202 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TFPI ELISA kit is to be used to detect and quantify protein levels of endogenous TFPI. The assay recognizes human TFPI. An antibody specific for TFPI has been pre-coated onto the microwells. The TFPI protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for TFPI is added to detect the captured TFPI protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1
背景説明

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.


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