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Human TDP-43 ELISA Kit1 Publications

カタログ番号: KE00005

93,000円

（希望販売価格）

容量: 1 Kit (96 assays)

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概要

製品名:
Human TDP-43 ELISA Kit
試験方法:
1 X 96 well plate
測定サンプル対象:
Serum,Plasma
アッセイ方法:
Sandwich
感度:
5.0 pg/mL
測定範囲:
78.1-5000 pg/mL
測定可能動物種:
Human
適用アプリケーション:
Sandwich ELISA
回収率:
Sample TypeAverageRange
Human plasma 88% 75%-109%
IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2,335.4 52.9 2.3
2 20 623.9 19.3 3.1
3 20 269.9 13.2 4.9
InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2,178.4 171.5 7.9
2 24 550.5 52.8 9.6
3 24 265.7 13.1 4.9
製品概要
KE00005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TDP-43 ELISA kit is to be used to detect and quantify protein levels of endogenous TDP-43. The assay recognizes human TDP-43. An antibody specific for TDP-43 has been pre-coated onto the microwells. The TDP-43 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TDP-43 is added to detect the captured TDP-43 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性

保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
別名:
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43
背景説明

TARDBP was firstly found as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in Amyotrophic lateral sclerosis (ALS). This kit is used to quantify TDP-43 level in vivo.


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