SARS-CoV-2 N protein ELISA Kit0 Publications

カタログ番号: KE30007

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
SARS-CoV-2 N protein ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
38 pg/mL

測定範囲:
375-6000 pg/mL

測定可能動物種:

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 116% 95%-127%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 3011.9 119.3 4.0
2 20 693.7 38.5 5.5
3 20 327.1 22.5 6.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2936.3 76.7 2.6
2 24 682.9 30.1 4.4
3 24 326.6 22.9 7.0

製品概要
KE30007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SARS-CoV-2 N protein ELISA kit is to be used to detect and quantify protein levels of endogenous SARS-CoV-2 N protein. The assay recognizes human SARS-CoV-2 N protein. An antibody specific for SARS-CoV-2 N protein has been pre-coated onto the microwells. The SARS-CoV-2 N protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SARS-CoV-2 N protein is added to detect the captured SARS-CoV-2 N protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
N protein, nucleocapsid phosphoprotein, SARS-CoV-2 N protein
背景説明

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.


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