Human Resistin ELISA Kit0 Publications

カタログ番号: KE00159

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human Resistin ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
15.2 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human Plasma 89% 79%-103%
Cell culture supernatants 86% 77%-96%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 977.2 35.8 3.7
2 20 216.5 10.9 5.0
3 20 51.5 3.7 7.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 961.4 47.4 4.9
2 24 224.2 10.5 4.7
3 24 52.3 3.3 6.2

製品概要
KE00159 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Resistin ELISA kit is to be used to detect and quantify protein levels of endogenous Resistin. The assay recognizes human Resistin. An antibody specific for Resistin has been pre-coated onto the microwells. The Resistin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Resistin is added to detect the captured Resistin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
ADSF, FIZZ3, HXCP1, resistin, RETN, RETN1, RSTN, XCP1
背景説明

Resistin, a cysteine-rich hormone secreted primarily by rodent fat cells, was postulated to be implicated in obesity, insulin resistance and diabetes. Resistin is also secreted by macrophages in humans, promoting inflammation (PMID: 20148674). Resistin modulates the synthesis and secretion of key pro-inflammatory cytokines such as TNF-α, IL-6 and IL12 in macrophages via a nuclear factor-kB (NF-kB)-dependent pathway. Given the connection between inflammation and cancer, resistin was soon considered for its role in cancer. There were observations supporting its possible use as a diagnostic marker, expression levels of which also seemed to differ in patients with varying clinical stages (PMID: 30317640). Resistin has been also linked to other pathologies such as atherosclerosis (PMID: 15710760).


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