Human/Mouse/Rat PIP5K1A ELISA Kit0 Publications

カタログ番号: KE00227

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human/Mouse/Rat PIP5K1A ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Human serum, Human plasma, Mouse serum, Rat serum and Cell lysate

アッセイ方法:
Sandwich

感度:
0.008 ng/mL

測定範囲:
0.156-10 ng/mL

測定可能動物種:
Human/Mouse/Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 87% 80%-98%
Mouse serum 102% 76%-120%
Rat serum 86% 78%-97%
Cell lysate 86% 75%-99%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 286.2 26.8 9.4
2 20 1,180.2 30.8 2.6
3 20 4,885.1 139.5 2.9

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 270.5 19.9 7.4
2 24 1,212.8 83.7 6.9
3 24 5,531.5 378.8 6.8

製品概要
KE00227 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PIP5K1A ELISA kit is to be used to detect and quantify protein levels of endogenous PIP5K1A. The assay recognizes human/mouse/rat PIP5K1A. An antibody specific for PIP5K1A has been pre-coated onto the microwells. The PIP5K1A protein in samples is captured by the coated antibody after incubation. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha
背景説明

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436).


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