Human PDGF-AB ELISA Kit

Sensitivity

14.5 pg/mL

Range

31.25 - 1000 pg/mL

Reactivity

Human

Cat no : KE00222

Synonyms

PDGF 1, PDGF A, PDGF AB, PDGF subunit A, PDGF1, PDGFA, PDGF-AB



Product Information

KE00222 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PDGF-AB ELISA kit is to be used to detect and quantify protein levels of endogenous PDGF-AB. The assay recognizes human PDGF-AB. An antibody specific for PDGF-AB has been pre-coated onto the microwells. The PDGF-AB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human PDGF-AB is added to detect the captured human PDGF-AB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human PDGF-AB ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 14.5 pg/mL
Range 31.25 - 1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum101%78%-122%
Cell culture supernatants113%93%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120425.326.86.3
22077.24.15.3
32041.82.35.4

InterAssay

Samplenmean ( pg/mL)SDCV%
124575.935.46.2
224127.99.37.3
32463.35.38.4

Background Information

Platelet-derived growth factor (PDGF) is one of the most abundant growth factors contained in the platelet alpha-granules and released upon platelet activation. It is also secreted by epithelial and mesenchymal cells. PDGF family consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. The PDGFR receptors are encoded by two genes: PDGFRA and PDGFRB. PDGFR is known to control growth, cell movement, angiogenesis and embryo development.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms PDGF 1, PDGF A, PDGF AB, PDGF subunit A, PDGF1, PDGFA, PDGF-AB