Human P21 ELISA Kit1 Publications

カタログ番号: KE00049

--

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human P21 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell lysates

アッセイ方法:
Sandwich

感度:
0.1 ng/mL

測定範囲:
0.25-16 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell lysates 87% 81%-97%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 10.29 0.201 2.0
2 20 3.05 0.070 2.3
3 20 0.63 0.025 4.0

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 10.24 0.346 3.4
2 24 3.08 0.094 3.1
3 24 0.67 0.052 7.8

製品概要
KE00049 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The P21 ELISA kit is designed for the quantitative determination of P21 in samples of human origin. An antibody specific for P21 has been pre-coated onto the microwells. The P21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for P21 is added to detect the captured P21 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1
背景説明

P21 (also known as CIP1, WAF1) is a cyclin-dependent kinase inhibitor. P21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of P21 is induced by wild-type but not mutant p53 protein, through which P21 mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.


Back
to top