Human MPO ELISA Kit

Sensitivity

9.1 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00171

Synonyms

MPO, myeloperoxidase



Product Information

KE00171 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MPO ELISA kit is to be used to detect and quantify protein levels of endogenous MPO. The assay recognizes Human MPO. An antibody specific for MPO has been pre-coated onto the microwells. The MPO protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MPO is added to detect the captured MPO protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human MPO ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Cell lysates, Saliva
Assay type Sandwich
Sensitivity 9.1 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum101%80%-126%
Cell culture supernatants97%81%-125%
Cell lysates105%91%-125%
saliva96%87%-110%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120974.419.52.0
220239.66.82.8
32050.94.07.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124961.536.43.8
224242.712.75.2
32453.44.48.3

Background Information

MPO(myeloperoxidase) is a peroxidase enzyme presented in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. Plasma concentration of MPO can be used as a specific marker of PMN activation. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). This enzymatic system plays an important role in human defense against microorganisms. The serum/plasma MPO levels have been associated with a variety of clinical conditions including inflammatory diseases, atherosclerosis, ischaemic stroke, hypertension, heart failure, risk of cardiovascular events and so on.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms MPO, myeloperoxidase