Human LGALS9/Galectin-9 ELISA Kit0 Publications

カタログ番号: KE00175

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human LGALS9/Galectin-9 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum,Plasma, Human milk, Urine

アッセイ方法:
Sandwich

感度:
3.9 pg/mL

測定範囲:
31.25-2000 pg/mL, 62.5-4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human milk 94% 74%-124%
Urine 95% 73%-108%
Serum 96% 83%-108%
Plasma 96% 78%-127%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 105.2 2.9 2.8
2 20 258.1 10.9 4.2
3 20 887.9 73.7 8.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 113.3 4.6 4.1
2 24 291.9 12.9 4.4
3 24 1,078.3 64.0 5.9

製品概要
KE00175 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LGALS9/Galectin-9 ELISA kit is to be used to detect and quantify protein levels of endogenous LGALS9/Galectin-9. The assay recognizes human LGALS9/Galectin-9. An antibody specific for LGALS9/Galectin-9 has been pre-coated onto the microwells. The LGALS9/Galectin-9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for LGALS9/Galectin-9 is added to detect the captured LGALS9/Galectin-9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21
背景説明

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.


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