Human IL-6R ELISA Kit

Sensitivity

0.1 pg/mL

Range

7.8 - 500 pg/mL

Reactivity

Human

Cat no : KE00118

Synonyms

CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80



Product Information

KE00118 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6R ELISA kit is to be used to detect and quantify protein levels of endogenous IL6R. The assay recognizes human IL6R. An antibody specific for IL6R has been pre-coated onto the microwells. The IL6R protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6R is added to detect the captured IL6R protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-6R ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 0.1 pg/mL
Range 7.8 - 500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3570

Recovery

Sample TypeAverageRange
Human serum89%80%-119%
Cell culture supernatants94%82%-108%
Urine97%80%-108%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120236.98.23.5
22061.61.82.9
32015.10.53.2

InterAssay

Samplenmean ( pg/mL)SDCV%
124271.412.94.8
22467.23.24.8
32416.00.85.2

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). Soluble form of IL-6R has been found in human serum and urine.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Cancer Lett

Induction of IL-6Rα by ATF3 enhances IL-6 mediated sorafenib and regorafenib resistance in hepatocellular carcinoma.

Authors - Zichan Dai
human

Mol Med

IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturition

Authors - Wen-Jia Lei

Front Immunol

E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism

Authors - Man Luo