Human IL-6 ELISA Kit


3.8 pg/mL


15.6-1000 pg/mL



Cat no : KE00007



Product Information

KE00007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL6 is added to detect the captured IL6 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IL-6 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 3.8 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3569


Sample TypeAverageRange
Cell culture supernatants107%99%-114%
Human plasma92%78%-122%


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms IL6


SpeciesSample TypeTitle

Cell Metab

Deamidation Shunts RelA from Mediating Inflammation to Aerobic Glycolysis.

Authors - Jun Zhao

Sci Signal

The suppressive effects of Mer inhibition on inflammatory responses in the pathogenesis of LPS-induced ALI/ARDS.

Authors - Masahiko Fukatsu

J Nutr Biochem

Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats.

Authors - Priscila Haygert

Cell Prolif

Long non-coding RNA 01126 promotes periodontitis pathogenesis of human periodontal ligament cells via miR-518a-5p/HIF-1α/MAPK pathway.

Authors - Mi Zhou
humancell culture supernatants

Eur J Med Chem

The discovery, design and synthesis of potent agonists of adenylyl cyclase type 2 by virtual screening combining biological evaluation.

Authors - Guowei Xu

J Clin Periodontol

Periodontitis and systemic markers of neurodegeneration: A case-control study.

Authors - Yago Leira


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kasper (Verified Customer) (08-27-2019)

Human IL-6 Elisa kit was used to determine IL-6 levels in cell culture supernatants as per the instructions. The kit worked quite well and was very easy to use.

  • Applications: Other,
  • Cell Tissue Type: cell culture supernatant