AuthentiKine™ Human IL-10 ELISA Kit2 Publications

カタログ番号: KE00170

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
AuthentiKine™ Human IL-10 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
plasma, serum, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
2.3 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
human plasma 92% 79%-100%
Cell culture supernatants 102% 88%-128%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 67.8 4.5 6.7
2 20 175.0 10.0 5.7
3 20 926.2 42.2 4.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 193.9 17.6 9.1
2 24 432.4 38.9 9.0
3 24 1108.4 111.3 10.0

製品概要
KE00170 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF
背景説明

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.


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