AuthentiKine™ Human IL-4 ELISA Kit0 Publications

カタログ番号: KE00232

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
AuthentiKine™ Human IL-4 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
1.4 pg/mL

測定範囲:
15.6-500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
human plasma 79 70-114
Cell culture supernatants 99 83-123

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 108.93 6.05 5.6
2 20 204.09 11.31 5.5
3 20 442.97 13.98 3.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 108.82 3.65 3.4
2 24 226.91 12.24 5.4
3 24 458.76 20.82 4.5

製品概要
KE00232 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-4 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-4. The assay recognizes human IL-4. An antibody specific for IL-4 has been pre-coated onto the microwells. The IL-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-4 is added to detect the captured IL-4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, interleukin 4, Pitrakinra
背景説明

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.


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