製品名:

Human IFN-gamma ELISA Kit

試験方法:

1 X 96 well plate

測定サンプル対象:

Serum, Plasma, Cell culture supernatants

アッセイ方法:

Sandwich

感度:

1.0 pg/mL

測定範囲:

15.6-1000 pg/mL

測定可能動物種:

Human

適用アプリケーション:

Sandwich ELISA

回収率:

Sample Type	Average	Range
Human plasma	95%	86%-102%
Cell culture supernatants	111%	84%-121%

製品概要

KE00063 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes human IFN-gamma. A monoclonal antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

保存方法:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:

IFI, IFn, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma