Human HNRNPA2B1 ELISA Kit

Sensitivity

5.0 pg/mL

Range

39-2500 pg/mL

Reactivity

Human

Cat no : KE00269

Synonyms

DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1



Product Information

KE00269 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HNRNPA2B1 ELISA kit is to be used to detect and quantify protein levels of endogenous HNRNPA2B1. The assay recognizes human HNRNPA2B1. An antibody specific for HNRNPA2B1 has been pre-coated onto the microwells. The HNRNPA2B1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HNRNPA2B1 is added to detect the captured HNRNPA2B1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human HNRNPA2B1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 5.0 pg/mL
Range 39-2500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3181

Recovery

Sample TypeAverageRange
Human plasma91%84%-98%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,025.429.02.8
220245.95.52.2
32055.22.03.7

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,042.136.13.5
224244.87.53.1
32457.05.59.7

Background Information

HNRNPA2B1 is one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport.HNRNPA2B1 is widely expressed in various tissues, and it's reported that it is highly expressed in many tumors. HNRNPA2B1 promotes tumor cell growth by activating the STAT3 pathway, regulating apoptosis and autophagy. HNRNPA2B1 is elevated in multiple myeloma patients and negatively correlated with favorable prognosis. It is found to activate cyclooxygenase-2 and promote tumor growth in human lung cancer.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1