AuthentiKine™ Human HGF ELISA Kit0 Publications

カタログ番号: KE00168

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
AuthentiKine™ Human HGF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.26 ng/mL

測定範囲:
0.313-20 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human Serum 91% 73%-112%
Human plasma 91% 73%-112%
Cell culture supernatants 117% 93%-126%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 4.7 0.30 6.4
2 20 9.3 0.65 7.0
3 20 18.3 0.92 5.0

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 5.1 0.42 8.4
2 24 10.5 0.96 9.2
3 24 20.9 19.3 9.2

製品概要
KE00168 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HGF ELISA kit is to be used to detect and quantify protein levels of endogenous HGF. The assay recognizes human HGF. An antibody specific for HGF has been pre-coated onto the microwells. The HGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, the horseradish peroxidase (HRP)-conjugated detection antibody specific for HGF is added to detect the captured HGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
背景説明

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa alpha-subunit (containing four kringle domains) and the 34 kDa beta-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.


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