Human EGFR ELISA Kit0 Publications

カタログ番号: KE00142

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human EGFR ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants, Cell lysates, Human milk

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
46.9-3000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 96% 74%-111%
Human milk 102% 91%-113%
Cell lysates 97% 81%-114%
Cell culture supernatants 89% 76%-97%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1265.9 19.6 1.6
2 20 322.7 9.5 2.9
3 20 79.9 1.7 2.1

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1249.3 94.5 7.6
2 24 313.6 20.2 6.5
3 24 76.2 6.6 8.6

製品概要
KE00142 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The EGFR ELISA kit is to be used to detect and quantify protein levels of endogenous EGFR. The assay recognizes human EGFR. An antibody specific for EGFR has been pre-coated onto the microwells. The EGFR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for EGFR is added to detect the captured EGFR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1
背景説明

The epidermal growth factor receptor (EGFR) belongs to the ErbB family of receptor tyrosine kinases (RTKs) and exerts critical functions in epithelial cell physiology. EGFR is a receptor of tyrosine kinase (RTK). It is consisted of a C-terminus intracellular region that possesses the kinase activity, and an N-terminus extracellular ligand-binding site, a hydrophobic transmembrane domain. EGFR is frequently mutated and/or overexpressed in different types of human cancers and is the target of multiple cancer therapies currently adopted in the clinical practice. Mutations in this gene are associated with lung cancer.


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