Human ECM1 ELISA Kit0 Publications

カタログ番号: KE00141

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human ECM1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
78.1-5000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 99% 81%-114%
Cell culture supernatants 85% 71%-96%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2,305.5 46.3 2.0
2 20 615.7 24.9 4.1
3 20 116.9 9.4 8.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2,070.5 108.7 5.3
2 24 572.8 29.9 5.2
3 24 152.8 15.9 10.4

製品概要
KE00141 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ECM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ECM1. The assay recognizes human ECM1. An antibody specific for ECM1 has been pre-coated onto the microwells. The ECM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for ECM1 is added to detect the captured ECM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
ECM1, extracellular matrix protein 1, Secretory component p85
背景説明

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers. It can be detected in human serum. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies.


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