AuthentiKine™ Human Cystatin C ELISA Kit
Cat no : KE00150
Synonyms
ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin
Validation Data Gallery
Product Information
KE00150 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Cystatin C ELISA kit is to be used to detect and quantify protein levels of endogenous Cystatin C. The assay recognizes human Cystatin C. An antibody specific for Cystatin C has been pre-coated onto the microwells. The Cystatin C protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Cystatin C is added to detect the captured Cystatin C protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human Cystatin C ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 10.5 pg/mL |
| Range | 15.6 -500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1471 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89% | 76%-95% |
| Cell culture supernatants | 86% | 73%-94% |
| Urine | 97% | 81%-107% |
| Saliva | 89% | 81%-98% |
| Human milk | 94% | 87%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 108.1 | 8.3 | 7.6 |
| 2 | 20 | 203.8 | 16.4 | 8.0 |
| 3 | 20 | 348.3 | 23.5 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 100.3 | 6.2 | 6.2 |
| 2 | 24 | 211.9 | 18.7 | 8.8 |
| 3 | 24 | 370.6 | 26.2 | 7.1 |
Background Information
Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Crit Care A translational study of Galectin-3 as an early biomarker and potential therapeutic target for ischemic-reperfusion induced acute kidney injury. |