Human CD138/Syndecan-1 ELISA Kit0 Publications

カタログ番号: KE00234

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CD138/Syndecan-1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Human serum, plasma

アッセイ方法:
Sandwich

感度:
0.01 ng/mL

測定範囲:
0.188-12 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 93.5% 69%-119%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 6.07 0.42 6.9
2 20 1.34 0.07 5.5
3 20 0.31 0.04 11.4

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 4.78 0.46 9.6
2 24 1.26 0.12 9.7
3 24 0.35 0.02 5.8

製品概要
KE00234 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD138 ELISA kit is to be used to detect and quantify protein levels of endogenous CD138. The assay recognizes human CD138. An antibody specific for CD138 has been pre-coated onto the microwells. The CD138 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for CD138 is added to detect the captured CD138 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD138, SDC, SDC1, SYND1, syndecan, syndecan 1
背景説明

CD138, also known as Syndecan-1 (SDC1), is a member of the transmembrane heparan sulfate proteoglycan family. CD138 acts as an extracellular matrix receptor and participates in many cellular functions, including cell-cell adhesion and cell-matrix adhesion. CD138 is expressed on plasma cells and various epithelial cell types. Altered CD138 expression has been described in many different tumor types. CD138 can be shed form the cell surface and exist as a soluble form, which has paracrine and autocrine functions and functions as competitor of transmembrane CD138 for growth factors and other extracellular ligands.


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