Human OX40/CD134 ELISA Kit0 Publications

カタログ番号: KE00209

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human OX40/CD134 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
5.8 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 98% 84%-110%
Human plasma 107% 98%-118%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 79.1 2.8 3.6
2 20 290.5 9.1 3.1
3 20 1,270.6 67.0 5.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 68.9 5.8 8.4
2 24 278.3 25.4 9.1
3 24 1,144.6 59.9 5.2

製品概要
KE00209 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The OX40 ELISA kit is to be used to detect and quantify protein levels of endogenous OX40. The assay recognizes human OX40. An antibody specific for OX40 has been pre-coated onto the microwells. The OX40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for OX40 is added to detect the captured OX40 protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
ACT35, ACT35 antigen, CD134, OX40, OX40L receptor, TNFRSF4, TXGP1L
背景説明

OX40 (CD134) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, OX40 is activated by its cognate ligand OX40L (CD134L) and functions as a T cell co-stimulatory molecule. OX40-OX40L interactions have been proposed as a potential therapeutic target for treating autoimmunity. OX40 can interact with TRAF2, TRAF3, and TRAF5. In addition to a membrane bound form, OX40 also exists as a soluble form, released by activated lymphocytes.


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