Human CCL4 ELISA Kit0 Publications

カタログ番号: KE00152

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CCL4 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
3.4 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 94% 75%-121%
Cell culture supernatants 93% 74%-112%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 436.0 38.1 8.7
2 20 102.2 9.8 9.6
3 20 60.6 5.5 9.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 417.7 28.1 6.7
2 24 115.5 5.7 5.0
3 24 62.2 6.7 10.7

製品概要
KE00152 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL4. The assay recognizes human CCL4. An antibody specific for CCL4 has been pre-coated onto the microwells. The CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL4 is added to detect the captured human CCL4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
ACT 2, ACT2, CCL4, MIP 1 beta
背景説明

CCL4(C-C chemokine ligand 4), also known as MIP-1β, plays a key role in inflammation and immune regulation. CCL4 is produced by a wide variety of cells including: immune cells (monocytes, B and T cells), fibroblasts, endothelial, and epithelial cells. CCL4 has a broad spectrum of target cells including immature dendritic cells, which express the cognate receptor CCR5. Increased expression of CCL4 was reported in the monocytes and blood of elderly individuals. Serum concentrations of CCL4 have been found to be significantly higher in patients with head and neck squamous cell carcinoma compared with controls.


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