Human CCL20/MIP-3 alpha ELISA Kit1 Publications

カタログ番号: KE00149

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CCL20/MIP-3 alpha ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.9 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 94% 74%-127%
Cell culture supernatants 94% 70%-129%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 171.7 5.6 3.3
2 20 42.6 0.8 1.8
3 20 9.8 0.3 3.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 176.0 6.0 3.4
2 24 42.2 0.8 1.8
3 24 10.4 0.4 3.6

製品概要
KE00149 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL20 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL20. The assay recognizes human CCL20. An antibody specific for CCL20 has been pre-coated onto the microwells. The CCL20 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL20 is added to detect the captured human CCL20 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Beta chemokine exodus 1, C C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, MIP 3 alpha, MIP 3a, MIP3A, SCYA20, Small inducible cytokine A20, ST38
背景説明

CCL20, also known as MIP-3 alpha, is a chemokine expressed mainly in the lymphatic tissue, lung and liver produced by cells related with inflammation and autoimmune response such as endothelial cells, neutrophils, natural killer (NK), Th17 cells and B cells among others. CCL20 contributes to inflammatory cell recruitment. CCL20 selectively signals through its receptor CCR6. The ligand-receptor pair CCL20/CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T cells and B cells, and also serves a role in the skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathological processes, such as those associated with cancer and rheumatoid arthritis.


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