Human BMP7 ELISA Kit
Cat no : KE00127
Synonyms
BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1
Validation Data Gallery
Product Information
KE00127 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BMP7 ELISA kit is to be used to detect and quantify protein levels of endogenous BMP7. The assay recognizes human BMP7. An antibody specific for BMP7 has been pre-coated onto the microwells. The BMP7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human BMP7 is added to detect the captured human BMP7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human BMP7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 655 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 79%-123% |
| Cell culture supernatants | 84% | 77%-92% |
| Urine | 95% | 79%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 517.9 | 30.8 | 5.9 |
| 2 | 20 | 125.8 | 6.6 | 5.3 |
| 3 | 20 | 30.7 | 2.2 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 399.7 | 37.7 | 9.4 |
| 2 | 24 | 99.5 | 7.8 | 7.8 |
| 3 | 24 | 27.3 | 2.1 | 7.7 |
Background Information
BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 is a glycosylated disulfide-linked 36 kDa homodimeric protein, having role in the induction, development, and regulation of adipocytes, especially brown adipose tissue (BAT). BMP7 is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation. Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival. Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Mol Sci Effect of Inducible BMP-7 Expression on the Osteogenic Differentiation of Human Dental Pulp Stem Cells. |