Human Angiopoietin 1 ELISA Kit0 Publications

カタログ番号: KE00212

--

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human Angiopoietin 1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Saliva

アッセイ方法:
Sandwich

感度:
17.3 pg/mL

測定範囲:
62.5 - 4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 98% 80%-116%
Human saliva 89% 71%-109%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2,021.5 94.5 4.7
2 20 557.8 13.4 2.4
3 20 155.8 6.6 4.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,845.2 59.9 3.2
2 24 544.9 20.9 3.8
3 24 161.1 5.9 3.6

製品概要
KE00212 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 1. The assay recognizes human Angiopoietin 1. An antibody specific for Angiopoietin 1 has been pre-coated onto the microwells. The Angiopoietin 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Angiopoietin 1 is added to detect the captured human Angiopoietin 1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003
背景説明

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer and other tumors.


Back
to top