Human APOE ELISA Kit0 Publications

カタログ番号: KE00156

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human APOE ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Human serum, Urine, Saliva, Human cerebrospinal fluid

アッセイ方法:
Sandwich

感度:
29.4 pg/mL

測定範囲:
78.1-5000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 100% 81%-118%
Urine 113% 99%-123%
Saliva 105% 77%-125%
Human cerebrospinal fluid 81% 73%-96%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2,811.2 88.6 3.2
2 20 776.0 23.9 3.1
3 20 291.0 7.4 2.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2,846.8 200.8 7.1
2 24 729.6 22.3 3.1
3 24 283.9 11.1 3.9

製品概要
KE00156 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOE ELISA kit is to be used to detect and quantify protein levels of endogenous APOE. The assay recognizes human APOE. An antibody specific for APOE has been pre-coated onto the microwells. The APOE protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for APOE is added to detect the captured APOE protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
AD2, Apo E, APOE, apolipoprotein E, LPG
背景説明

APOE (apolipoprotein E) is a plasma protein implicated in lipid metabolism. It is produced predominantly by hepatocytes, macrophages, and neural cells. In the CNS, APOE is the major extracellular lipid carrier and plays a key role in neuronal protection/repair after injury. APOE is polymorphic, with three major alleles: APOE2 (cys112, cys158), APOE3 (cys112, arg158), and APOE4 (arg112, arg158). APOE2 plays a protective role against both Alzheimer's (AD) and heart disease. APOE4 confers a higher risk for atherosclerosis and Alzheimer's disease. Analysis of cerebrospinal fluid (CSF) and plasma APOE protein levels in AD patients had been performed to examine its association with AD pathology. In old age, high plasma APOE levels precede an increase of circulating CRP and strongly associates with cardiovascular mortality.


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