Human APOB ELISA Kit0 Publications

カタログ番号: KE00158

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human APOB ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum,Plasma, Cell Culture supernatants, Human milk

アッセイ方法:
Sandwich

感度:
0.68 ng/mL

測定範囲:
3.9-250 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human Serum 95% 77%-121%
Cell Culture supernatants 103% 86%-122%
Human milk 102% 75%-122%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 119.9 4.1 3.4
2 20 28.3 0.7 2.6
3 20 7.2 0.4 5.4

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 133.3 9.8 7.3
2 24 31.0 2.4 7.9
3 24 8.0 0.8 10.0

製品概要
KE00158 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOB ELISA kit is to be used to detect and quantify protein levels of endogenous APOB. The assay recognizes human APOB. An antibody specific for APOB has been pre-coated onto the microwells. The APOB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human APOB is added to detect the captured human APOB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB
背景説明

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB gene encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. APOB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. APOB-48 and APOB-100 share a common N-terminal sequence, but APOB-48 lacks APOB-100's C-terminal LDL receptor binding region. Plasma APOB levels are associated with coronary heart disease.


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