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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Hsc70 Monoclonal antibody

Hsc70 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A4B3

Cat no : CL594-66442

Synonyms

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL594-66442

    IF Staining of HEK-293 using CL594-66442

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 Hsc70 antibody (CL594-66442, Clone: 1A4B3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy HSC70/HSPA8 Ready-To-Use IHC Kit. HSC70/HSPA8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66442-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66442 targets Hsc70 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hsc70 fusion protein Ag17910 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 8
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007276
Gene symbol HSPA8
Gene ID (NCBI) 3312
RRIDAB_2919992
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

Protocols

Product Specific Protocols
IF protocol for CL594 Hsc70 antibody CL594-66442Download protocol
Standard Protocols
Click here to view our Standard Protocols