Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, rat heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
15700-1-AP targets HSPB7 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8328 Product name: Recombinant human HSPB7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC006319 Sequence: MSHRTSSTFRAERSFHSSSSSSSSSTSSSASRALPAQDPPMEKALSMFSDDFGSFMRPHSEPLAFPARPGGAGNIKTLGDAYEFAVDVRDFSPEDIIVTTSNNHIEVRAEKLAADGTVMNTFAHKCQLPEDVDPTSVTSALREDGSLTIRARRHPHTEHVQQTFRTEIKI 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 27kDa protein family, member 7 (cardiovascular) |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 18-23 kDa |
| GenBank accession number | BC006319 |
| Gene Symbol | HSPB7 |
| Gene ID (NCBI) | 27129 |
| RRID | AB_2233322 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPB7 (Heat shock protein beta-7), also known as cardiovascular heat shock protein (cvHsp), is a unique member of the small heat shock protein (sHSP) family. HSPB7 is predominantly expressed in the heart and skeletal muscle, with much lower levels found in the aorta and adipose tissue. HSPB7 plays a specialized role in maintaining cellular structure and homeostasis rather than acting primarily as a protein-folding chaperone.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSPB7 antibody 15700-1-AP | Download protocol |
| IP protocol for HSPB7 antibody 15700-1-AP | Download protocol |
| WB protocol for HSPB7 antibody 15700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun Homozygous expression of the myofibrillar myopathy-associated p.W2710X filamin C variant reveals major pathomechanisms of sarcomeric lesion formation. | ||
Exp Cell Res Isoform-specific functions of synaptopodin-2 variants in cytoskeleton stabilization and autophagy regulation in muscle under mechanical stress. | ||
Int J Oncol Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation. | ||
J Alzheimers Dis Psychometric criteria for superior cognitive performance in very old adults. |