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  • Featured Product
  • KD/KO Validated

GRP75 Polyclonal antibody

GRP75 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14887-1-AP

Synonyms

CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

Validation Data Gallery

Applications:
  • WB analysis using 14887-1-AP

    WB analysis using 14887-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 cells using 14887-1-AP

    WB analysis of A549 cells using 14887-1-AP

    A549 cells (shcontrol and shRNA of GRP75)were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:500.

  • WB analysis of mouse testis using 14887-1-AP

    WB analysis of mouse testis using 14887-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14887-1-AP

    IP experiment of mouse brain using 14887-1-AP

    IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:2000) with mouse brain tissue lysate 3680ug.

  • IP experiment of NIH/3T3 using 14887-1-AP

    IP experiment of NIH/3T3 using 14887-1-AP

    IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

  • IHC staining of human breast cancer using 14887-1-AP

    IHC staining of human breast cancer using 14887-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14887-1-AP (GRP75 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPA9/GRP75 antibody included
  • IF Staining of HepG2 using 14887-1-AP

    IF Staining of HepG2 using 14887-1-AP

    Immunofluorescent analysis of HepG2 cells using 14887-1-AP (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of HepG2 using 14887-1-AP

    IF Staining of HepG2 using 14887-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP75 antibody (14887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 14887-1-AP

    FC experiment of HepG2 using 14887-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug GRP75 antibody (14887-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHT-29 cells, A549 cells, mouse testis tissue, Jurkat cells, NIH/3T3 cells, mouse liver
Positive IP detected inmouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

14887-1-AP targets GRP75 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP75 fusion protein Ag6673 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 9 (mortalin)
Calculated molecular weight 74 kDa
Observed molecular weight 74-75 kDa
GenBank accession numberBC000478
Gene symbol HSPA9
Gene ID (NCBI) 3313
RRIDAB_2120458
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

Protocols

Product Specific Protocols
WB protocol for GRP75 antibody 14887-1-APDownload protocol
IHC protocol for GRP75 antibody 14887-1-APDownload protocol
IF protocol for GRP75 antibody 14887-1-APDownload protocol
IP protocol for GRP75 antibody 14887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
View Article
humanWB

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin
View Article
mouse

EMBO Mol Med

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

Authors - Masayuki Ide
View Article
mouseWB

J Cell Biol

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

Authors - Anne Korwitz
View Article
ratWB

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang
View Article
humanWB

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Christina (Verified Customer) (11-14-2023)

I used this antibody for HeLa extracts in Western Blot and worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HeLa cells
FH

Ning (Verified Customer) (02-27-2023)

Specifically probe endogenous GRP75 with a right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
GRP75 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14887-1-AP)
FH

Staci (Verified Customer) (02-02-2023)

Antibody works very well with GRP75. Very little non-specific binding on immunoblot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse Islet protein lysates