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KD/KO Validated

HSPA1L Polyclonal antibody

Name: HSPA1L Polyclonal antibody
Brand: Proteintech
SKU: 13970-1-AP

HSPA1L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13970-1-AP

Synonyms

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

Validation Data Gallery

Applications:

AllWBIPIHCIF

WB analysis of mouse liver using 13970-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 13970-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13970-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13970-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13970-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human saliva using 13970-1-AP
human saliva were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 13970-1-AP
IP result of anti-HSPA1L (IP:13970-1-AP, 4ug; Detection:13970-1-AP 1:1000) with HepG2 cells lysate 1800ug.
IHC staining of human endometrial cancer using 13970-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human endometrial cancer using 13970-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 13970-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 13970-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 13970-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSPA1L antibody (13970-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 13970-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, MCF-7 cells, HeLa cells, HepG2 cells, human saliva tissue, rat testis tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human endometrial cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 2 publications below

Product Information

13970-1-AP targets HSPA1L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSPA1L fusion protein Ag4995 相同性解析による交差性が予測される生物種
Full Name	heat shock 70kDa protein 1-like
Calculated molecular weight	70 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC034483
Gene symbol	HSPA1L
Gene ID (NCBI)	3305
RRID	AB_2119675
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)

Protocols

Product Specific Protocols
WB protocol for HSPA1L antibody 13970-1-AP	Download protocol
IHC protocol for HSPA1L antibody 13970-1-AP	Download protocol
IF protocol for HSPA1L antibody 13970-1-AP	Download protocol
IP protocol for HSPA1L antibody 13970-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne) Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring. Authors - Xiaomei Liu View Article
human	WB	Virol J Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. Authors - Yuting Yang View Article
mouse	WB,IF	PeerJ The heat shock protein family gene Hspa1l in male mice is dispensable for fertility. Authors - Xin Wang KO Validated View Article
mouse	IF	Neurobiol Dis SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function Authors - Mukesh Gautam View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPA1L Polyclonal antibody

HSPA1L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of mouse liver using 13970-1-AP

WB analysis of rat testis using 13970-1-AP

WB analysis of MCF-7 using 13970-1-AP

WB analysis of HeLa using 13970-1-AP

WB analysis of HepG2 using 13970-1-AP

WB analysis of human saliva using 13970-1-AP

IP experiment of HepG2 using 13970-1-AP

IHC staining of human endometrial cancer using 13970-1-AP

IHC staining of human endometrial cancer using 13970-1-AP

IHC staining of human breast cancer using 13970-1-AP

IHC staining of human breast cancer using 13970-1-AP

IF Staining of HepG2 using 13970-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.

Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9.

The heat shock protein family gene Hspa1l in male mice is dispensable for fertility.

SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function