Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 10979-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10979-1-AP (GRP94 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10979-1-AP
WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells..
WB analysis of K-562 using 10979-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10979-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10979-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10979-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10979-1-AP
IP result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.
IHC staining of human breast cancer using 10979-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 10979-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10979-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10979-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of human appendicitis using 10979-1-AP
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse pancreas using 10979-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of U2OS using 10979-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of MCF-7 using 10979-1-AP
1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug GRP94 Polyclonal antibody (10979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 10979-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

HSP90B1, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, GP96

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human colon tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse pancreas tissue, human appendicitis tissue
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10979-1-AP targets GRP94 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1439 Product name: Recombinant human GRP94 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC009195 Sequence: MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAARRR 相同性解析による交差性が予測される生物種
Full Name	heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight	96 kDa
Observed molecular weight	94 kDa
GenBank accession number	BC009195
Gene Symbol	GRP94
Gene ID (NCBI)	7184
RRID	AB_2119057
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14625
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 10979-1-AP	Download protocol
IHC protocol for GRP94 antibody 10979-1-AP	Download protocol
IF protocol for GRP94 antibody 10979-1-AP	Download protocol
IP protocol for GRP94 antibody 10979-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. Authors - Na Liu View Article
pig,human	WB	Vet Microbiol ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. Authors - Liang Zhang View Article
rat	WB	Mol Med Rep Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats. Authors - Maojun Liu View Article
mouse	WB,IHC,IF	J Immunol Res Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis Authors - Qiaohui Hong View Article
human	WB	Carcinogenesis Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis Authors - Huajie Zong View Article
human	WB	Exp Brain Res CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis Authors - Danyang Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10979-1-AP

WB analysis of HEK-293 using 10979-1-AP

WB analysis of K-562 using 10979-1-AP

WB analysis of NIH/3T3 using 10979-1-AP

WB analysis of MCF-7 using 10979-1-AP

WB analysis of A549 using 10979-1-AP

IP experiment of MCF-7 using 10979-1-AP

IHC staining of human breast cancer using 10979-1-AP

IHC staining of mouse brain using 10979-1-AP

IHC staining of human colon using 10979-1-AP

IHC staining of human lung cancer using 10979-1-AP

IF Staining of human appendicitis using 10979-1-AP

IF Staining of mouse pancreas using 10979-1-AP

IF Staining of U2OS using 10979-1-AP

FC experiment of MCF-7 using 10979-1-AP

GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis

Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis

CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis