HSD11B2 Polyclonal antibody
HSD11B2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14192-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, mouse kidney tissue, human kidney tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IHC | See 6 publications below |
| IF | See 1 publications below |
Product Information
14192-1-AP targets HSD11B2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSD11B2 fusion protein Ag5146 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxysteroid (11-beta) dehydrogenase 2 |
| Calculated molecular weight | 405 aa, 44 kDa |
| Observed molecular weight | 40-43 kDa |
| GenBank accession number | BC036780 |
| Gene symbol | HSD11B2 |
| Gene ID (NCBI) | 3291 |
| RRID | AB_2119643 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD11B2 antibody 14192-1-AP | Download protocol |
| IHC protocol for HSD11B2 antibody 14192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nutrients Maternal High-Fructose Corn Syrup Intake Impairs Corticosterone Clearance by Reducing Renal 11β-Hsd2 Activity via miR-27a-Mediated Mechanism in Rat Offspring | ||
Oncotarget Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats. | ||
Ecotoxicol Environ Saf Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse. | ||
Carcinogenesis Modulation of the oral glucocorticoid system during black raspberry mediated oral cancer chemoprevention.
| ||
Sci Rep Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets. | ||
Brain Res Response of the maternal hypothalamus to cold stress during late pregnancy in rats. |
