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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HRG Polyclonal antibody

HRG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26252-1-AP

Synonyms

histidine rich glycoprotein, HPRG, HRG, HRGP

Validation Data Gallery

Applications:
  • WB analysis of human plasma using 26252-1-AP

    WB analysis of human plasma using 26252-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 26252-1-AP (HRG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 26252-1-AP

    IHC staining of mouse liver using 26252-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26252-1-AP (HRG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 26252-1-AP

    IHC staining of mouse liver using 26252-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26252-1-AP (HRG antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

26252-1-AP targets HRG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRG fusion protein Ag16278 相同性解析による交差性が予測される生物種
Full Name histidine-rich glycoprotein
Calculated molecular weight 525 aa, 60 kDa
Observed molecular weight70 kDa
GenBank accession numberBC069574
Gene symbol HRG
Gene ID (NCBI) 3273
RRIDAB_2880447
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HRG antibody 26252-1-APDownload protocol
IHC protocol for HRG antibody 26252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Front Pharmacol

17β-Estradiol Enhances Schwann Cell Differentiation via the ERβ-ERK1/2 Signaling Pathway and Promotes Remyelination in Injured Sciatic Nerves.

Authors - Yun Gu
View Article