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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated HPGD Monoclonal antibody

HPGD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F1F5

Cat no : CL594-66798

Synonyms

15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL594-66798

    IF Staining of A549 using CL594-66798

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 HPGD antibody (CL594-66798, Clone: 3F1F5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66798-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66798 targets HPGD in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HPGD fusion protein Ag1499 相同性解析による交差性が予測される生物種
Full Name hydroxyprostaglandin dehydrogenase 15-(NAD)
Calculated molecular weight 29 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC018986
Gene symbol HPGD
Gene ID (NCBI) 3248
RRIDAB_2920040
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 HPGD antibody CL594-66798Download protocol
Standard Protocols
Click here to view our Standard Protocols