Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, MDA-MB-453s cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
15448-1-AP targets HOXC8 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, chicken, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7675 Product name: Recombinant human HOXC8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC053898 Sequence: MSSYFVNPLFSKYKAGESLEPAYYDCRFPQSVGRSHALVYGPGGSAPGFQHASHHVQDFFHHGTSGISNSGYQQNPCSLSCHGDASKFYGYEALPRQSLYGAQQEASVVQYPDCKSSANTNSSEGQGHLNQNSSPSLMFPWMRPHAPGRRSGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKLPGARDEEKVEEEGNEEEEKEEEEKEENKD 相同性解析による交差性が予測される生物種 |
| Full Name | homeobox C8 |
| Calculated molecular weight | 27 aa, 7 kDa |
| Observed molecular weight | 33-38 kDa |
| GenBank accession number | BC053898 |
| Gene Symbol | HOXC8 |
| Gene ID (NCBI) | 3224 |
| RRID | AB_2878140 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31273 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HOXC8 belongs to highly conserved subgroup of 39 genes housed into four clusters HOXA-HOXD within the superfamily of homeobox genes. HOX genes encode homeodomain- transcription factors that are responsible for regulating morphogenesis through axial patterning during embryogenesis. Homeobox C8 (HOXC8) is a transcription factor preferentially overexpressed in a large percentage of non-small cell lung carcinoma (NSCLC). HOXC8 participates NSCLC development by controlling CASP1 expression and pyroptosis (PMID: 40701951).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXC8 antibody 15448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res N6-methyladenosine-modified circSLCO1B3 promotes intrahepatic cholangiocarcinoma progression via regulating HOXC8 and PD-L1 | ||
iScience DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis | ||
Oncogenesis HOXC8 promotes proliferation and migration through transcriptional up-regulation of TGFβ1 in non-small cell lung cancer.
| ||
iScience Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. | ||
Mol Carcinog Upregulation of MGP by HOXC8 promotes the proliferation, migration, and EMT processes of triple-negative breast cancer.
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