Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10871-1-AP targets HOOK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HOOK1 fusion protein Ag1311 相同性解析による交差性が予測される生物種 |
| Full Name | hook homolog 1 (Drosophila) |
| Calculated molecular weight | 85 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC011621 |
| Gene Symbol | HOOK1 |
| Gene ID (NCBI) | 51361 |
| RRID | AB_2120812 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJC3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HOOK1 antibody 10871-1-AP | Download protocol |
| IHC protocol for HOOK1 antibody 10871-1-AP | Download protocol |
| IF protocol for HOOK1 antibody 10871-1-AP | Download protocol |
| IP protocol for HOOK1 antibody 10871-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes. | ||
Elife Cylicins are a structural component of the sperm calyx being indispensable for male fertility in mice and human |