Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 10578-1-AP
WB result of HNRNPUL1 antibody (10578-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPUL1 transfected HEK-293 cells.
WB analysis of A549 using 10578-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10578-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10578-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10578-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10578-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10578-1-AP
IP result of anti-HNRNPUL1 (IP:10578-1-AP, 5ug; Detection:10578-1-AP 1:2000) with HeLa cells lysate 2000ug.
IHC staining of human ovary tumor using 10578-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:100 (under 25x lens).
IF Staining of HepG2 using 10578-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10578-1-AP (HNRNPUL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10578-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPUL1 antibody (10578-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10578-1-AP
Immunofluorescent analysis of HepG2 cells, using HNRNPUL1 antibody 10578-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

HNRNPUL1 Polyclonal antibody

HNRNPUL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10578-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

Heterogeneous nuclear ribonucleoprotein U-like protein 1, E1B-AP5, E1BAP5, E1B-55 kDa-associated protein 5, E1B AP5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, MCF-7 cells, HEK-293 cells, mouse brain tissue, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 12 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

Product Information

10578-1-AP targets HNRNPUL1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0899 Product name: Recombinant human HNRNPUL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC002564 Sequence: MDVRRLKVNELREELQRRGLDTRGLKAELAERLQAALEAEEPDDERELDADDEPGRPGHINEEVETEGGSELEGTAQPPPPGLQPHAEPGCYSGPDGHYAMDNITRQNQFYDTQVIKQENESGYERRPLEMEQQQAYRPEMKTEMKQGAPTSFLPPEASQLKPDRQQFQSRKRPYEENRGRGYFEHREDRRGRSPQPPAEEDEDDFDDTLVAIDTYNCDLHFKVARD 相同性解析による交差性が予測される生物種
Full Name	heterogeneous nuclear ribonucleoprotein U-like 1
Calculated molecular weight	120 kDa
Observed molecular weight	90-115 kDa
GenBank accession number	BC002564
Gene Symbol	HNRNPUL1
Gene ID (NCBI)	11100
RRID	AB_2120655
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BUJ2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .

Protocols

Product Specific Protocols
IF protocol for HNRNPUL1 antibody 10578-1-AP	Download protocol
IHC protocol for HNRNPUL1 antibody 10578-1-AP	Download protocol
IP protocol for HNRNPUL1 antibody 10578-1-AP	Download protocol
WB protocol for HNRNPUL1 antibody 10578-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing Authors - Qi Wen Chen View Article
human	WB,IF,IP	Proc Natl Acad Sci U S A Influenza virus targets the mRNA export machinery and the nuclear pore complex. Authors - Satterly Neal N View Article
	WB	PLoS Pathog Cellular RNA Binding Proteins NS1-BP and hnRNP K Regulate Influenza A Virus RNA Splicing. Authors - Tsai Pei-Ling PL View Article
human	WB,IF	EMBO Rep Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. Authors - Chakraborty Papia P View Article
mouse	WB,IF	J Autoimmun RNA-binding protein hnRNP UL1 binds κB sites to attenuate NF-κB-mediated inflammation. Authors - Zhongfei Ma KO Validated View Article
human	IHC	Exp Cell Res HNRNPUL1 inhibits cisplatin sensitivity of esophageal squamous cell carcinoma through regulating the formation of circMAN1A2. Authors - Juan Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 10578-1-AP

WB analysis of A549 using 10578-1-AP

WB analysis of MCF-7 using 10578-1-AP

WB analysis of HEK-293 using 10578-1-AP

WB analysis of HeLa using 10578-1-AP

WB analysis of mouse brain using 10578-1-AP

IP experiment of HeLa using 10578-1-AP

IHC staining of human ovary tumor using 10578-1-AP

IF Staining of HepG2 using 10578-1-AP

IF Staining of HepG2 using 10578-1-AP

IF Staining of HepG2 using 10578-1-AP

HNRNPUL1 Polyclonal antibody

HNRNPUL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Influenza virus targets the mRNA export machinery and the nuclear pore complex.

Cellular RNA Binding Proteins NS1-BP and hnRNP K Regulate Influenza A Virus RNA Splicing.

Vesicular stomatitis virus inhibits mitotic progression and triggers cell death.

RNA-binding protein hnRNP UL1 binds κB sites to attenuate NF-κB-mediated inflammation.

HNRNPUL1 inhibits cisplatin sensitivity of esophageal squamous cell carcinoma through regulating the formation of circMAN1A2.