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  • KD/KO Validated

HNRNPA1 Polyclonal antibody

HNRNPA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11176-1-AP

Synonyms

Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1



Tested Applications

Positive WB detected inHeLa cells, mouse colon tissue, K-562 cells, Neuro-2a cells, C6 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPA1 Ready-To-Use IHC Kit. HNRNPA1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11176-1-AP targets HNRNPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivity human, rat, mouse, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPA1 fusion protein Ag1656 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A1
Calculated molecular weight 34 kDa
Observed molecular weight 34, 40 kDa
GenBank accession numberBC009600
Gene symbol HNRNPA1
Gene ID (NCBI) 3178
RRIDAB_2117177
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

Protocols

Product Specific Protocols
WB protocol for HNRNPA1 antibody 11176-1-APDownload protocol
IHC protocol for HNRNPA1 antibody 11176-1-APDownload protocol
IF protocol for HNRNPA1 antibody 11176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
mouseWB

Mol Cell

Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Authors - Jing Wen
humanWB

Mol Cell

A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.

Authors - Jin-Zhou Huang
  • KD Validated
WB

Nat Commun

Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.

Authors - Wen-Juan Li
  • KD Validated
pigWB

Mol Biol Evol

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.

Authors - Ke Chen
humanWB,IF

J Cell Biol

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.

Authors - Joseph R Tran

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhongwen (Verified Customer) (09-26-2023)

Good quality. It is easy for you to find the target bind.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
HNRNPA1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11176-1-AP)
FH

Katie (Verified Customer) (04-08-2022)

Works nicely for IF in primary CD4+ T cells. 2hr Primary incubation at 37 and 2hr secondary incubation at 37oc 1:1000.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50