Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, Raji cells, K-562 cells, mouse spleen tissue, hTERT-RPE1 cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 2 publications below |
Product Information
14817-1-AP targets HMOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6575 Product name: Recombinant human HMOX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-293 aa of BC002396 Sequence: MSAEVETSEGVDESEKKNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHYIGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKAFEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGSSCPFRTAMAVLRK 相同性解析による交差性が予測される生物種 |
| Full Name | heme oxygenase (decycling) 2 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC002396 |
| Gene Symbol | HMOX2 |
| Gene ID (NCBI) | 3163 |
| RRID | AB_2118843 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| IHC protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| IF protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| IP protocol for HMOX2 antibody 14817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. | ||
Acta Pharm Sin B Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage. | ||
Oxid Med Cell Longev Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion. | ||
Am J Physiol Gastrointest Liver Physiol Carbon monoxide produced by HO-1 upregulation is the main factor behind the abnormal motility seen in experimental ulcerative colitis in mice | ||
Cells Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions. |