Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Raji using 14670-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14670-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14670-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 14670-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 14670-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 14670-1-AP
IP result of anti-HLA-F (IP:14670-1-AP, 4ug; Detection:14670-1-AP 1:3000) with A431 cells lysate 1040 ug.
IHC staining of human tonsillitis using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.
IHC staining of human spleen using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.
IHC staining of human skin cancer using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin cancer using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.
IHC staining of human placenta using 14670-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.

Featured Product
KD/KO Validated

HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14670-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, human placenta tissue, human spleen tissue, A431 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human tonsillitis tissue, human placenta tissue, human skin cancer tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 7 publications below
IF	See 1 publications below

Product Information

14670-1-AP targets HLA-F in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6335 Product name: Recombinant human HLA-F protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-298 aa of BC062991 Sequence: AGSHSLRYFSTAVSRPGRGEPRYIAVEYVDDTQFLRFDSDAAIPRMEPREPWVEQEGPQYWEWTTGYAKANAQTDRVALRNLLRRYNQSEAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADTVAQITQRFYEAEEYAEEFRTYLEGECLELLRRYLENGKETLQRADPPKAHVAHHPISDHEATLRCWALGFYPAEITLTWQRDGEEQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPQPLILRWEQS 相同性解析による交差性が予測される生物種
Full Name	major histocompatibility complex, class I, F
Calculated molecular weight	39 kDa
Observed molecular weight	40-45 kDa
GenBank accession number	BC062991
Gene Symbol	HLA-F
Gene ID (NCBI)	3134
RRID	AB_1519280
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P30511
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Protocols

Product Specific Protocols
IP protocol for HLA-F antibody 14670-1-AP	Download protocol
WB protocol for HLA-F antibody 14670-1-AP	Download protocol
IHC protocol for HLA-F antibody 14670-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IHC	Kidney Int Upregulation of HLA-F expression by BK polyomavirus infection induces immune recognition by KIR3DS1-positive natural killer cells. Authors - Tobias F Koyro View Article
human	IHC	Int J Cancer Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma. Authors - Zhang X X View Article
		J Immunol Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans. Authors - Louis Marcellin View Article
mouse,human	WB	Int J Biol Sci Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis. Authors - Xin Chen KD Validated View Article
human	WB,IHC	Lung Cancer HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer. Authors - Lin Aifen A View Article
human	IHC	Oncol Lett Lesion human leukocyte antigen-F expression is associated with a poor prognosis in patients with hepatocellular carcinoma. Authors - Yongfu Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Raji using 14670-1-AP

WB analysis of human placenta using 14670-1-AP

WB analysis of A431 using 14670-1-AP

WB analysis of Raji using 14670-1-AP

WB analysis of human spleen using 14670-1-AP

IP experiment of A431 using 14670-1-AP

IHC staining of human tonsillitis using 14670-1-AP

IHC staining of human tonsillitis using 14670-1-AP

IHC staining of human spleen using 14670-1-AP

IHC staining of human spleen using 14670-1-AP

IHC staining of human skin cancer using 14670-1-AP

IHC staining of human skin cancer using 14670-1-AP

IHC staining of human placenta using 14670-1-AP

IHC staining of human placenta using 14670-1-AP

HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Upregulation of HLA-F expression by BK polyomavirus infection induces immune recognition by KIR3DS1-positive natural killer cells.

Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma.

Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer.

Lesion human leukocyte antigen-F expression is associated with a poor prognosis in patients with hepatocellular carcinoma.