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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human HLA-DR (L243)

HLA-DR Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

L243

Cat no : CL488-65218

Synonyms

HLA DRA, HLA DRB, HLA-DR, HLA-DRA, HLA-DRB, MHC class II antigen DR

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CL488-65218

    FC experiment of human PBMCs using CL488-65218

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human HLA-DR (CL488-65218, Clone:L243), or unstained. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL488-65218

    FC experiment of human PBMCs using CL488-65218

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human HLA-DR (CL488-65218, Clone:L243), or unstained. Cells were not fixed. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65218-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65218 targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
RRIDAB_2883222
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 HLA-DR antibody CL488-65218Download protocol
Standard Protocols
Click here to view our Standard Protocols