Validation Data Gallery
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Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 1 publications below |
Product Information
CL488-65218 targets HLA-DR in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class II, DR alpha |
| Calculated molecular weight | 254 aa, 29 kDa |
| GenBank accession number | BC032350 |
| Gene Symbol | HLA-DRA |
| Gene ID (NCBI) | 3122 |
| ENSEMBL Gene ID | ENSG00000204287 |
| RRID | AB_2883222 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01903 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 HLA-DR antibody CL488-65218 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
