Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)ChIP

WB analysis using 16047-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16047-1-AP
WB result of Histone H4 antibody (16047-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H4 transfected HEK-293 cells.
WB analysis of MCF-7 using 16047-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16047-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16047-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16047-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16047-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16047-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16047-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 16047-1-AP
rat thymus tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 16047-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16047-1-AP
IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.
IP experiment of HeLa using 16047-1-AP
IP result of anti-Histone H4 (IP:16047-1-AP, 4ug; Detection:16047-1-AP 1:300) with HeLa cells lysate 2680ug.
IHC staining of mouse small intestine using 16047-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 16047-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 16047-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16047-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16047-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 16047-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 16047-1-AP
Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 16047-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 16047-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 16047-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
ChIP experiment of HEK-293 using 16047-1-AP
Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

Featured Product
KD/KO Validated

Histone H4 Polyclonal antibody

Histone H4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Cat No. 16047-1-AP

Publications(72)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Synonyms

HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (72)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HepG2 cells, HT-1080 cells, MCF-7 cells, mouse kidney tissue, mouse thymus tissue, rat thymus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse small intestine tissue, mouse colon tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells
Positive ChIP detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Chromatin immunoprecipitation (ChIP)	CHIP :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 63 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 3 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below
RIP	See 1 publications below

Product Information

16047-1-AP targets Histone H4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8999 Product name: Recombinant human Histone H4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-103 aa of BC012587 Sequence: MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG 相同性解析による交差性が予測される生物種
Full Name	histone cluster 1, H4e
Calculated molecular weight	102 aa, 11 kDa
Observed molecular weight	14 kDa, 11 kDa
GenBank accession number	BC012587
Gene Symbol	Histone H4
Gene ID (NCBI)	8367
RRID	AB_2118625
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62805
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protocols

Product Specific Protocols
IF protocol for Histone H4 antibody 16047-1-AP	Download protocol
IHC protocol for Histone H4 antibody 16047-1-AP	Download protocol
IP protocol for Histone H4 antibody 16047-1-AP	Download protocol
WB protocol for Histone H4 antibody 16047-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping. Authors - Lilien N Voong KD Validated View Article
human	WB	Cell Res Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation. Authors - Xianteng Wang View Article
mouse	WB	Cell Res SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation. Authors - Fang Yu View Article
		Mol Cell An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma. Authors - Jin Zhou View Article
rat	WB	Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Authors - Megha Jhanji View Article
human	WB	Autophagy The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy. Authors - Liangde Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16047-1-AP

WB analysis of HEK-293 using 16047-1-AP

WB analysis of MCF-7 using 16047-1-AP

WB analysis of HeLa using 16047-1-AP

WB analysis of HT-1080 using 16047-1-AP

WB analysis of HepG2 using 16047-1-AP

WB analysis of mouse kidney using 16047-1-AP

WB analysis of HepG2 using 16047-1-AP

WB analysis of HepG2 using 16047-1-AP

WB analysis of rat thymus using 16047-1-AP

WB analysis using 16047-1-AP

IP experiment of HeLa using 16047-1-AP

IP experiment of HeLa using 16047-1-AP

IHC staining of mouse small intestine using 16047-1-AP

IHC staining of mouse colon using 16047-1-AP

IHC staining of mouse colon using 16047-1-AP

IHC staining of human breast cancer using 16047-1-AP

IHC staining of human breast cancer using 16047-1-AP

IHC staining of mouse small intestine using 16047-1-AP

IF Staining of HeLa using 16047-1-AP

IF Staining of HeLa using 16047-1-AP

IF Staining of HeLa using 16047-1-AP

FC experiment of HeLa using 16047-1-AP

ChIP experiment of HEK-293 using 16047-1-AP

Histone H4 Polyclonal antibody

Histone H4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.

Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.

SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation.

An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.