Histone H4 Polyclonal antibody

HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HT-1080 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MCF7 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

rat thymus tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.

IP result of anti-Histone H4 (IP:16047-1-AP, 4ug; Detection:16047-1-AP 1:300) with HeLa cells lysate 2680ug.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.