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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HINT1 Monoclonal antibody

HINT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G3E4

Cat no : CL488-67583

Synonyms

FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-67583

    IF Staining of HeLa using CL488-67583

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 HINT1 antibody (CL488-67583, Clone: 1G3E4 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67583-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67583 targets HINT1 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HINT1 fusion protein Ag16662 相同性解析による交差性が予測される生物種
Full Name histidine triad nucleotide binding protein 1
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC007090
Gene symbol HINT1
Gene ID (NCBI) 3094
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HINT1 antibody CL488-67583Download protocol
Standard Protocols
Click here to view our Standard Protocols