Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IHC | See 6 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10717-1-AP targets HINT1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1142 Product name: Recombinant human HINT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-126 aa of BC007090 Sequence: MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG 相同性解析による交差性が予測される生物種 |
| Full Name | histidine triad nucleotide binding protein 1 |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC007090 |
| Gene Symbol | HINT1 |
| Gene ID (NCBI) | 3094 |
| RRID | AB_2248272 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49773 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HINT1 antibody 10717-1-AP | Download protocol |
| IP protocol for HINT1 antibody 10717-1-AP | Download protocol |
| WB protocol for HINT1 antibody 10717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells. | ||
Oncogene Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells. | ||
J Med Chem A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide | ||
Cell Cycle The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells. | ||
Pharmacogenomics J Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence. | ||
